cDNA Synthesis Protocol by Reverse Transcription cdna technology

by autisticchiaNGMAI



Hey Friends,
cDNA (complementary DNA) is synthesized from an RNA template. This is done by Reverse Transcription. In this example, the oligo d(T)-primers are used to prime the cDNA synthesis. An alternative would be to use random hexamers as primers. cDNA is synthesized before qPCR to check gene expression levels.

qPCR:

References:
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Cheers
Henrik .

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cDNA Synthesis Protocol by Reverse Transcription

cDNA Synthesis Protocol by Reverse Transcription

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cDNA Synthesis Protocol by Reverse Transcription
cdna technology
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26 comments

Fantasy Sehun 21/11/2021 - 8:29 Chiều

i was confused for intron thingy and u made it easy

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Beatriz Vieira 21/11/2021 - 8:29 Chiều

I was so confused with these terms and you explained so clearly and so well. Thank you so much

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MUHAMMAD ANEES 21/11/2021 - 8:29 Chiều

Why cant we use mRNA directly in microarray or qPCR

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Jeff Stoker 21/11/2021 - 8:29 Chiều

Man trying to play God not our place to mess with God's code that makes us human.

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Praveen M 21/11/2021 - 8:29 Chiều

Thank you so much sir❤

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john tindell 21/11/2021 - 8:29 Chiều

Thank you so much. The explanation is very intelligible.

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ahmad omari 21/11/2021 - 8:29 Chiều

amazing , keep going

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It's My Bio 21/11/2021 - 8:29 Chiều

Thank You So much Sir.. I watched lot of Videos But No one explained about RNaseH Function and I was really confused. You Really Explained very nicely. Thanks for making this video..

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debre markos university vine 21/11/2021 - 8:29 Chiều

Thank u so much. before I watch this video I read some what notes but they were boring. ur video were so interesting with smooth sound and full information.

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Sanjana Chaudhary 21/11/2021 - 8:29 Chiều

Thank you soooo much for this.
This thing was more like rocket science to me before watching this video. Cleared all the concepts in just one go.
Thanks again. 😊
Have great day ahead

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Zenyatta The Peaceful 21/11/2021 - 8:29 Chiều

Excellent video. You made this concept easy to understand, thank you.

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ATTILA HORVATH 21/11/2021 - 8:29 Chiều

Wait a second people…forgive my lack of understanding but i dont understand why start to syntettize anything about and HUMAN DNA??? Why want to know what GOD did once and why and how and how to REVERSE IT/////?????!! Please someone explain..Many thanks

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Mario Garrote Filho 21/11/2021 - 8:29 Chiều

Fantastic! Amazing! Incredible! Astounding! Mighty! Wonder! Super!

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Abhinav Kumar 21/11/2021 - 8:29 Chiều

Thank you so much Henrik's Lab. You are doing a very noble job by providing us such useful concept clearing video. Through your videos we are able to understand the topic better than hours of classroom learning. ❤❤

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Juana Montoya Venegas 21/11/2021 - 8:29 Chiều

Muy buen video! Gracias!

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Sarah mahmoud 21/11/2021 - 8:29 Chiều

Thank you very much..

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Седа Гаспарян 21/11/2021 - 8:29 Chiều

thank you very very much!

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Gabrielle Haddad 21/11/2021 - 8:29 Chiều

Thanks!!!!

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Vitor Fernando 21/11/2021 - 8:29 Chiều

Amazing! Thanks!

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J DA 21/11/2021 - 8:29 Chiều

This is great. Perfect rythm. Thank you.

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Kevin Jones 21/11/2021 - 8:29 Chiều

Is there a way we could translate all the proteins in a human genome and create a lab created human since the entire genome has been sequenced?

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Li Cheuk Hin 21/11/2021 - 8:29 Chiều

in 2:57 may I ask how whole mrna are replaced by the DNA? thank you

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Sabin Acharya 21/11/2021 - 8:29 Chiều

Hello, i am facing problem in cdna synthesis. would you please help me to know your protocol?

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Flo Sto 21/11/2021 - 8:29 Chiều

Bist du deutscher ? So hörst du dich an XD

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elvis 21/11/2021 - 8:29 Chiều

Sehr verständlich, vielen Dank!

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Sabin Acharya 21/11/2021 - 8:29 Chiều

Thank you. How can we contact you if we get problem while doing qpcr wirk?

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